I have managed to get sisters back together. It wasn't easy but with patience it worked.
The sisters were about 5 and 6 years old, not siblings - same Dad. We had sunshine and light all those years and within the pack which fluctuated up and down around the 6 mark. But someone came into the garden through a gate and sparked off a humdinger of a fight. One girl was hanging from the back of my hand, tearing flesh and ligaments so I needed stitches.
We kept them crated in the kitchen, within sight, sound and smell of each other until their wounds healed. Then I would get one girl out and take her down to the road, out of the garden while Marvin brought the other. Outside 'their' property - it is always best to do things on neutral territory - Marvin handed me the second girl and I walked them a few hundred yards at first then longer, one in each hand and taking care not to let them come too close to each other. When we got back to the garden, Marvin would take one girl and, each holding one, with lots of voice encouragement and treats, we'd let them approach each other. At first they were firmly on leads and were escorted back to the kitchen and their crates.
We did this four or even five times every day.
Over time, we found they would actually sniff the same blade of grass while on the walk and behave, even off lead, back in the garden.
So we took a deep breath and let them both back into the house without leads. And back into the pack - no longer crated.
It never occurred to me that I would fail to re-integrate them and as for spaying either of them, NO WAY !
They lived in harmony thereafter - one making 10.5 years and the other 16.5.
My hand healed nicely too !